COMPARISON OF CULTURE WITH POLYMERASE CHAIN REACTION FOR DETECTION OF UREAPLASMA UREALYTICUM IN ENDOCERVICAL SPECIMENS

Background: Ureaplasma urealyticum is known as a sexually transmitted agent, causing mainly urethritis, pelvic int1ammatory disease, spontaneous abortion, pyelonephritis, infertility, stillbirth, low birth weight neonatal meningititis, and neonatal pneumonia. U. urealyticum infections not only jeopardize fertility but also pose a risk for infertility treatment and resulting pregnancies. Diagnosis of U. urealyticul7I infections by bacterial conventional methods is very difficult The aim of this study was to compare culture with polymerase chain reaction (PCR) to determine the prevalence of U. urealyticum in endocervical specimens from infertile women. Methods: 312 endocervical swab samples were taken from infel1ile women, and transpOJ1ed with mycoplasma transpol1 media. The culture was done with liquid-solid methods. DNA was extracted by Cadieux method, and analyzed by PCR protocol with species-specific U4&US primers. Results: U. urealyticum was detected in 26.2% (82/312) of specimens by both culture and PCR methods. 12.5% (39/312) of samples were PCR positive as well as culture positive, 11.2% (351312) were positive only by PCR,and2.S%(8/312) were positive only by culture. Conclusion: A sensitivity of 90% and 57% was found for PCR and culture respectively, PCR is therefore sensitive and more rapid «24 hour) than culture (2-5 days) for the detection of U. urealyticum in endocervical secretions.